INCIDE

University of Konstanz
IEEE International Conference on Image Processing

Stem cell microscopic image segmentation using supervised normalized cuts

X. Huang, C. Li, M. Shen, K. Shirahama, J. Nyffeler, M. Leist, M. Grzegorzek, O. Deussen
Teaser of Stem cell microscopic image segmentation using supervised normalized cuts

Material

Paper (.pdf, 1.4 MB)

Abstract

A vast amount of toxicological data can be obtained from feature analysis of cells treated in vitro. However, this requires microscopic image segmentation of cells. To this end, we propose a new strategy, namely Supervised Normalized Cut Segmentation (SNCS), to segment cells that partially overlap and have a large amount of curved edges. SNCS approach is a machine learning based method, where loosely annotated images are used first to train and optimise parameters, and then the optimal parameters are inserted into a Normalized Cut segmentation process. Furthermore, we compare our segmentation results using SNCS to another four classical and two state-of-the-art methods. The overall experimental result shows the usefulness and effectiveness of our method over the six comparison methods.

BibTeX

@inproceedings{Huang2016Stemcellmicroscopic,
  address    = {Piscataway, NJ},
  author     = {X. Huang and C. Li and M. Shen and K. Shirahama and J. Nyffeler and M. Leist and M. Grzegorzek and O. Deussen},
  booktitle  = {IEEE International Conference on Image Processing},
  doi        = {10.1109/ICIP.2016.7533139},
  editor     = {Karam, Lina},
  isbn       = {978-1-4673-9961-6},
  pages      = {4140--4144},
  publisher  = {IEEE},
  title      = {Stem cell microscopic image segmentation using supervised normalized cuts},
  year       = {2016},
  url        = {http://graphics.uni-konstanz.de/publikationen/Huang2016Stemcellmicroscopic},
}